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(b) Scatter plot of the normalized fold change of interacting PETs vs. fold change of accessibility at anchors for all the identified interactions.
(a) Scatter plot of the observed fold change of interacting PETs vs. fold change of accessibility at anchors for all the identified interactions.
Most identified interactions are novel and require further validation since they involved loosely characterized proteins.
It revealed L803 binding with a hydrophobic surface patch and identified interactions between Pro8 (L803) and Phe93 (GSK-3β).
To validate the model further, the identified interactions will be used for designing mutagenesis studies to investigate their effect on binding activity.
Chromosome conformation capture analysis identified interactions between the HOXD9 promoter and risk-associated SNPs at 2q31.1.
However, many identified interactions have not been documented, and therefore in many instances only likely effects were identified.
A biochemical screen identified interactions between SUN1 and SUN2 and DNA-dependent protein kinase (DNAPK) complex that functions in DNA nonhomologous end joining repair and possibly in DDR.
Only 5% are expected to be incorrectly identified interactions.
Fifth, many of the identified interactions were further examined by expressing these proteins in human cells.
Of these 79 identified interactions, 58 are novel (Table 2) while the other 21 have previously been reported (Table 1).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com