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One of these IgG γ chain cDNAs, (caIgG-A), represents 92.5% of the IgG γ chain cDNAs in a dog spleen cell cDNA library; a second partial IgG γ chain cDNA (caIgG-B) was also identified in the library.
Six Copia families were identified in the library.
Y, homologous UniGene cluster identified in the library.
Note: 1 Identified in the library 2 Not identified in the library Potential target genes of differentially expressed miRNAs in each comparison were predicted by miRanda [ 24].
The gene encoding 60S subunit of ribosmal protein (identified in the library) was used as control.
Y*, homologous UniGene cluster identified in the library with an occurrence >0.15%.
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Two clusters coding for putative astacin-like zinc metalloproteases were identified in the libraries.
Five clusters coding for putative chymotrypsins were identified in the libraries and each cluster was 5' truncated.
Candidate heat-shock responsive cod cDNAs identified in the libraries were further investigated using real-time quantitative reverse transcription - polymerase chain reaction (QPCR).
Cloning and expression in E. coli of six enzyme candidates identified in the libraries provided access to individual enzyme activities, which all proved to be coherent with the primary and secondary functional screens.
The following paragraphs give a detailed description of the most abundant sequences identified in the libraries and sequences that are of interest with respect to the midgut physiology and Leishmania life cycle.
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