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Within the diamond lattice itself, hydrogen-vacancy and hydrogen-nitrogen-vacancy complexes have been identified in negative charge states by electron paramagnetic resonance.
No mutations on the RAF1 gene were identified in negative cases of PTPN11, SOS1, and KRAS.
The m/z values matched the species identified in negative mode.
Next, proteins identified in negative controls or as abundant contaminants were excluded from consideration, leaving a smaller list of OMA-1-associated proteins (51 different proteins; Figure S8).
Table 1 constitutes proteins from the GFP-D-HURP AP-MS which: (i) either were not identified in negative controls or were identified in negative controls with scores of at least 4-fold less than in GFP-D-HURP and (ii) had an MS score of > 30 and (iii) had > 10% peptide:protein coverage.
No exon 20 mutation was identified in negative control MCF7 and BT474 cells, any patient tumor cells, or captured WBCs among our samples, although sequencing of BT20 cells (positive control) detected the expected exon 20 mutation at A3140G H1047RR), confirming the accuracy of our sequencing method.
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No pathogenic variants were identified in the negative controls.
Identical DNA sequences also were rarely identified in our negative extraction controls.
PC molecular species were identified in both negative and positive ionisation modes.
No intragenic deletions or duplications by MLPA were identified in patients negative to direct exon sequencing.
Our aim was to establish whether (1) micrometastases could be identified in histologically negative groin nodes and (2) whether there is an association between clinical recurrence and micrometastases.
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