In summary, the present study identified fructose as an ideal nutrient supplement that can be used to improve P. citrinum W1 growth and antifungal activity.
Among the eight sugars identified, fructose, glucose, mannose, 6-deoxy-mannose and sedoheptulose decreased, and only maltose was elevated.
Of the 371 contigs identified, only two sequences showed homology to previously identified fructose-bisphosphate aldolase and metallothionein type-II proteins from avocado, with the remaining 369 not having any avocado sequence homolog in the NR database.
TIGAR, which also known as the chromosome 5 open reading frame or C12orf5 in human, is a recently identified fructose-2,6-bisphosphatase that contributes to regulation of glucose metabolism [ 52].
As expected from a photosynthetic tissue four chloroplastidic proteins were identified: fructose-1,6-bisphosphatase (FBPase), phosphoribulokinase (PRK), ferredoxin-NADP reductase (FRN) and an hypothetical protein that belongs to the epimerase subfamily.
Two isoforms of aldolase were identified: fructose-bisphosphate aldolase A, and fructose-bisphosphate aldolase B. Aldolase A was identified three times, all within a few kD of the expected molecular weight of 40 kD, and at approximately the expected pI of 8.0.
It happened again when we "identified" high fructose corn syrup as the root of all evil.
Enzymes involved in the glycolysis pathway were also identified including fructose-bisphosphate aldolase (spot 566), triosephosphate isomerase (spot 503), phosphoglycerate kinase (spot 431), phosphoglycerate mutase (spot 531) and enolase (spot 18).
These results demonstrate the suitability of C. saccharobutylicum P262 for butanol production from unhydrolyzed plant inulin and also identify limited fructose utilization as a potential metabolic engineering target for improving the fermentability of unhydrolyzed inulin-rich plant extracts to butanol.
Now, my kids know how to identify high fructose corn syrup on a label and know that they shouldn't eat it.
Similar studies by Max and Chapados (2007) on IR waveband assignment for glucose and fructose identified endocyclic C-O bands around 1080 cm−1.
