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Moreover, mAb 12A localised and identified dendritic cells that present DRαβ1*0401/HC gp-39263 275 gp-39263 275 synovial tissue of DRαβ1*0401-positive RA patients.
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SIRPα expression has been used to identify dendritic cell subsets across species and here we examined its expression and function on intestinal DCs in mice.
Liver DC can be defined in broad terms, such as CD45+ cells with a high expression of major histocompatibility complex class II (MHCII) and the absence of other hematopoietic markers [ 11], but many markers are needed to identify dendritic cell subsets [ 12].
Local spot uncaging near identified dendritic spines produced EPSP-like potential (uEPSP) in the cell soma.
Palmitoylation of TLR2, which was uniquely identified in dendritic cells, was mapped to a transmembrane domain-proximal cysteine.
Expression of MHC II, CD40, CD80 and CD86 was used to identify activated dendritic cells.
CD83 is a type-I membrane protein and an efficient marker for identifying mature dendritic cells.
We therefore used the endolysosomal localization of our wild type fluorophore-tagged class II MHC molecules as a marker to identify immature dendritic cells.
As far as classification of cell types is concerned, we argue that the performed cluster analysis identified all dendritic cell types present in our sample.
A density-based cluster algorithm (Ankerst et al. 1999) identified 9 dendritic cell types (Fig. 2) and revealed objective criteria that distinguished between cell types (Supplementary Fig. S1 and, Table S1).
A recent study investigating the phenotype of in situ MV-infected cells in fatal measles cases identified macrophages and dendritic cells as predominant infected cells28.
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