Cell proliferation was determined by counting cells positive for Ki67 antigen, which identifies cycling cells, and reported in graph as percentage of positive cells (Fig. 4A).
We used our analytical methods to identify cycling mRNAs in the dataset.
Sequential sections were stained for Ki67 to identify cycling cells and exclude the possibility that biotinylated Mab F-E2G5 merely detects such cells.
To correlate the presence of SCCA with liver regeneration activity, consecutive slices of each tumour were stained with the Ki-67 equivalent, MIB-1 antibody, which identifies cycling cells in formalin-fixed tissue samples (Cattoretti et al, 1992).
To first analyze the proliferative effect, HaCaT cells were serum starved, treated with SNs from CHO-AFs or from control cells for 48 h at 37 °C and then fixed and stained with antibodies against Ki67 antigen, which identify cycling cells (Fig. 3a).
To investigate whether the decrease in cell proliferation was due to apoptosis of drug-treated cells, cells were stained with a combination of 7-AAD (to detect dead cells) and the proliferation marker Ki-67, and analysed by flow cytometry to identify cycling cells.
Only 149 of the 38,445 expressed transcript probes (0.39%) were positively identified as cycling (Table 1, Figure S1), an approximately 96% reduction in the number of detected cycling probes relative to that of leaves.
He failed the International Cycling Union's biological passport program and was one of three riders identified by cycling's governing body as having suspicious blood profiles.
Out of the 11,195 genes identified as cycling (Q value cutoff 0.8) under LDHH condition, 2,225 genes mapped to the RiceCyc metabolic network.
Only 47 genes were identified as cycling diurnally in ears and their amplitudes were attenuated severely compared with leaves.
