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Gene expression profiling of isolated aortic EC identified cell cycle and cell migration as the principal biological processes affected in the double mutant EC.
Because primary cells are sensitive to artificial treatment, we used techniques that identified cell cycle stage (rather than cell synchronization) to minimize the risk of interfering with normal accumulation (or loss) of transient modifications.
Many of the identified cell cycle genes were found to be associated with G2/M cell cycle checkpoint/arrest including Fen1, Cdc25b, Mbd4, Egr1, Ccnb1, Ccnb2, Plk4, Aurka, and Mad2L1.
A number of studies of Ts16, Ts65Dn and DS embryonic brain development have identified cell cycle and neurogenesis abnormalities in the neocortex and hippocampus [44], [45], [49], [50], [51].
Most of the identified cell cycle genes were up-regulated during callus induction.
Comparing benign osteoblastomas with osteosarcomas identified cell cycle regulation as the most prominently changed pathway.
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We also examined the wound response prognosis signature without including the initially identified cell cycle-associated genes [40] and the predictive signature for dasatinib treatment response subdivided for prostate and breast cancer [54].
In a study that was published previously [5], a genome-scale analysis identified cell cycle-regulated genes in the human genome by identifying those genes with common expression patterns.
Ebp1 is the human homologue of a previously identified cell cycle-regulated mouse protein p38-2G4.
DOI: http://dx.doi.org/10.7554/eLife.01630.012 We further identified cell cycle-regulated phosphorylated peptides that peak in abundance at different stages of the cell cycle.
ERBB3 binding protein 1 (EBP1) is the human homologue of a previously identified cell cycle-regulated mouse protein p38-2G4 [ 5].
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