These ENs, which can be identified by the expression of Dac, express low levels of Abd-B (Fig. 6C-D′).
In our culture system, the OECs phenotypically identified by the expression of αSMA were capable of expressing iNOS even in the absence of any stimulus (Fig. 5a,b).
The vessels, identified by the expression of CD31, were scored as mature when expressing the epitope LH39 in the basal membrane and as newly formed when expressing αVβ3 on the endothelial cells and/or lacking LH39 expression.
mDN axons, identified by the expression of tyrosine hydroxylase (TH, the rate-limiting enzyme of dopamine synthesis), express the Shh receptor component Smoothened (Smo; Figure 1B, 1C).
S. pneumoniae infection of OECs, phenotypically identified by the expression of P75NRT, was able to reduce the expression of iNOS (Fig. 5h,i) compared to uninfected cultures (Fig. 5f,g).
Exocrine differentiation occurs in the majority of the luminal compartment and is identified by the expression of the androgen receptor (AR), keratin 18 (K18) and prostate specific antigen (PSA).
Virus-induced gene silencing is being used to screen candidate genes for their involvement in MIA biosynthesis, and the function of selected genes can be identified by the expression and assay of recombinant proteins in bacterial or yeast systems.
Further applications of this approach include imaging of rare miR-expressing cells and positive regulation of a therapeutic or selector gene in target cells identified by the expression of selected miRs.
For example, platelet-derived microparticles are identified by the expression of CD41 and endothelial-derived microparticles are identified by their expression of CD146 [1].
Subsequent fate restriction of mesodermal precursors toward cardiovascular and hematopoietic progenitors was identified by the expression of Mesp1 and Flk1 (Wu et al., 2008).
Among the hCD45+ cells, human B cells, identified by the expression of CD19, were observed in all hNOK mice, whereas human T cells, identified by the expression of CD3, were observed in approximately 60% of the mice (Table 1).
