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Myocytes were identified by smooth muscle actin staining after one week in culture (Figure 6C), and were also observed to comprise approximately 30% ±10% of derivatives.
The Hartmannella was identified by smooth, spherical appearance) [ 16, 20].
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Colima ceramics can be identified by their smooth, round forms and their warm brown-red slip.
The underlying structure in the SNP variant frequency scatter plot of a given chromosome was identified by fitting smoothing splines in the generalized linear mixed model framework, as described previously [ 29].
In addition to PCR genotyping, mice were examined by immunohistochemistry for the presence of Cre and absence of AR in PTM cells, identified by immunoexpression of smooth muscle actin (SMA).
Smooth muscle cells of the tunica media of arteries were identified by detecting α-smooth muscle actin using a mouse monoclonal antibody (Sigma-Aldrich, Dorset, UK) at 1 4000 dilution.
In addition to PCR genotyping, mice were examined by immunohistochemistry for the presence of Cre and absence of AR in SV smooth muscle cells, identified by immunoexpression of α-smooth muscle actin (SMA), using a previously published method (44).
Small structures on processes of GFP expressing neurons were identified by subtracting a smoothed image from the original image and then analyzed similarly to puncta of synaptophysin-GFP, except that the size range was between 0.5 and 3 µm.
Myogenic differentiation was identified by increased expression of smooth muscle actin and calponin.
An increase of dermal myofibroblastic cells were identified by immunostains for the smooth muscle markers in roughly 85 70% of the biopsies with c-GVHD and SSc, respectively (Figure 2 E and F).
Smooth muscle cells in the walls of arteries were identified by staining for α-smooth muscle actin monoclonal antibody (Sigma-Aldrich, 1 200).
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