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Matheny, C. J. et al. Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.
In practice such cells containing clonally expanded mtDNA mutations may be identified by sequential cytochrome c oxidase/succinate dehydrogenase (COX/SDH) histochemistry.
IgE-binding proteins were identified by sequential addition of biotinylated rabbit antibody specific for human IgE (Vector, Peterborough, UK), Extravidin peroxidase complexes (Sigma, Poole, UK) and Super Signal West Pico chemiluminescent substrate (Thermo).
Identified by sequential linkage analysis of trait cosegregation in a full three-generation pedigree and F2 intercross mapping, a CORT-related QTL was detected on mouse chromosome 3 between 41.3 cM (closest marker: rs13477268 at 40.5 cM) and 43.3 cM (closest marker: rs4138887 at 45.3 cM) with a linkage peak at 42.3 cM.
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Copper release was minor and significantly lower than the mobile fraction identified by the sequential extraction (Fig. 1).
Likewise, abdominal compartment syndrome (ACS) was defined as IAP > 20 mmHg in combination with at least one new end-organ failure, which can be identified by a Sequential Organ Failure Assessment (SOFA) sub-score ≥ 3 [23].
Major vessels can readily be identified by following sequential 3-mm cuts.
Differentially expressed genes were identified by performing sequential pair-wise comparisons between the GFP+ and GFP− fractions from four independent sorting experiments using two different cell lines, performed in two different laboratories.
Likewise, abdominal compartment syndrome (ACS) was defined as IAP > 20 mmHg in combination with at least one new end-organ failure, which can be identified by a Sequential Organ Failure Assessment (SOFA) sub-score ≥ 3 [ 23].
Controls were identified by using sequential-digit dialing, a technique that uses sequential digits from the end of the telephone number of the case-patient to contact potential controls (11 ).
Controls were contacted and identified by using sequential-digit dialing beginning with the matching patient's telephone number.
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