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We have demonstrated that endothelial cells and osteoblasts (identified by cell morphology, quantification of specific marker antigens, calcium deposition and capillary-like growth) were able to differentiate and expand from donor rat bone marrow mononuclear cell populations.
Furthermore, only few cell types can be identified by cell morphology and specific antibodies for immunohistochemistry are available only for a limited range of proteins.
However, several important immunological pathways that may be common to the cervical cell population were identified by the gene expression analyses that would not have been identified by cell phenotyping alone.
hMSCs were isolated and identified by cell surface markers, then, cultured in Minimum Essential Medium Alpha Medium (α-MEM) supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin, and incubated in a humidified atmosphere of 5% CO2 at 37°C.
Having observed that SALL4 was enriched in HS/PCs that were identified by cell surface markers, we planned to evaluate the expression of this stem cell factor in SP and non-SP cells in various blood cancer cell lines (HL-60, NB4, KG1a and RPMI8226 cells), as well as investigate the role of SALL4 in drug resistance and cancer stem cells as identified by SP studies.
ASCs can be identified by cell surface markers that distinguish different B cell populations.
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Saito et al. [41] detected early cell alteration that was identified by cell-cycle and proliferation changes after irradiation exposure both in vitro and in vivo by using MEMRI.
In all fluorescent activated cell sorter (FACS) experiments, cells were pre-coated with anti mouse CD16/CD32 (BD Pharmingen,) to block unspecific binding, and T cells were identified by cell-surface labeling with APC-labeled anti-Thy1.2 (BD Pharmingen).
Human pDCs can be identified by cell-surface expression of MHC molecules, the α-chain of the IL-3 receptor (CD123), and the presence of blood dendritic-cell (DC) antigens known as BDCA2 and BDCA4 in a proportion of cells [ 1].
Similar to natural bone mineral, hydroxyapatite (HA) is relatively easier to be identified by cells or biomacromolecules, which can improve the bioactivity, bioavailability, and biocompatibility of scaffolds.
In normal human bone marrow, p53 protein was not detected in the proliferative, progenitor cell populations identified by the cell surface antigens CD34 (progenitor cells of multiple lineages) or glycophorin (erythroid precursors).
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