It is tempting to hypothesize that our five genetic clusters identified by the assignment tests correspond to five different introductions, but the situation is certainly more complex.
To identify genomic segments potentially contributing to local adaptation, we divided samples into the two primary clusters (the Eastern and Western populations) and six clusters identified by genetic assignment in STRUCTURE and calculated FST (Weir and Cockerham 1984).
We estimated the Nb/Nc ratios by dividing Nb by either (i) the number of anadromous individuals counted at the dam or (ii) the number of breeders identified by parental assignments, including mature male parr.
Divergent selection for length at two summers of age was performed on G2 fish, and response to selection was evaluated by comparing the offspring of the selected sires in G3, in a communal test where genetic groups were identified by microsatellite parentage assignment.
Multi-dose markers were identified by comparing chromosomal assignment of markers across populations.
Compounds were identified by de novo assignment of spin systems in H-C HSQC and HMBC, and H-H magnitude COSY experiments, and identifications were confirmed by spectral comparison to authentic compounds.
Averaging the q for the six TESS clusters for the three altitude classes, we observed that the previous cline of variation along altitude identified by the STRUCTURE assignment was also maintained for TESS; indeed, at low altitudes, the T1 and T2 clusters were mainly present, and these tended to be substituted by the T5 and T6 clusters at high altitudes.
The placebo and the active product will be identical in appearance and will be identified by a code, the assignment of which will be unknown by the patients and the investigators.
In the case of weak signals where the ompA genotype could not be accurately identified by the software, the assignment was done manually based on the closest match: these cases were termed "weak positive".
The turkey genome physical map was constructed based on 74,013 BAC fingerprints (11.9 × coverage) from two independent libraries, and it was integrated with the turkey genetic map and chicken genome sequence using over 41,400 BAC assignments identified by 3,499 overgo hybridization probes along with > 43,000 BAC end sequences.
