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21 significant proteins were identified bound to Transferrin.
Additionally, WT1 protein was identified bound to several of these gene promoters in native chromatin of transfected LNCaP cells.
Furthermore, a peptide from the AaxC transporter of this strain was identified bound to MHC class I molecules from infected murine dendritic cells [ 40].
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Because the peptides that we identified bound tightly to Mcl-1 and showed good specificity for Mcl-1 over other human Bcl-2 family members, we chose to develop them further as Mcl-1 binders.
For each dataset we identified bound regions according to a False Discovery Rate (FDR) model using the TiMAT software (http://bdtnp.lbl.gov/TiMAT/TiMAT2/; summary of dataset analysis in Table S1).
For each data set, we identified bound regions (peaks) according to a false discovery rate (FDR) model using Ringo software [ 23].
Western blot analysis was performed on the extracts used for pull-down assay and the eluted fractions to identify bound proteins.
Although, methodologically, this study had good internal validity, it has limited generalisability because it was not possible to identify, bound, or sample the target population accurately.
To identify bound regions, and their peak positions, binding ratios exceeding the genomic average by at least three standard deviations were extracted.
Indeed, a number of diterpenes were identified that bound to recombinant Dopamine receptors of the 2 subtype (D2 receptors) which are present in pituitary lactotropes and which mediate the inhibitory effects of dopamine and dopaminergic drugs on pituitary prolactin release.
The binding assays revealed that all of the identified peptides bound to IAb with significant affinities in the range of 34 – 1858 nM (Table 2).
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