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The kidney was the first site identified as activating 25(OH D by 1α-hydroxylation, forming hormonal 1,25-dihydroxyvitamin D (calcitriol), which promotes calcium absorption and bone calcification.
One of the genes was identified as activating transcription factor 3 (Athat, thad han an induced alternative splice variant at all three studies time points, while the constitutive, full-length variant was low expressed and did not change with the treatment.
For example a prediction stating that atoms {1, 2, 3} and bonds {1, 2, 3} are identified as ACTIVATING with no other relationships found would identify that the removal of those atoms and bonds would result in the query structure no longer being considered active.
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Subsequently, microparticles positive for E-selectin (CD62E), which originated from activated endothelial cells were identified as activated EMPs.
Other pathway identified as activated by cAMP includes another guanine nucleotide exchange protein called CNrasGEF, which directly activates Ras [33].
We analyzed those genes identified as activated by shear force [30], cyclic pressure [31], hydrostatic pressure [32] and mechanical stress [33].
All of the genes identified as activated in the serum response, with the exception of CTGF, show no significant upregulation in gene expression in our datasets.
Voxels surpassing a statistical threshold of p = 0.05 (t-contrast analysis, corrected for multiple comparisons, height threshold t = 3) were identified as activated.
Hypertrophied and bushy Iba-1-positive cells were identified as activated microglia.
Importantly, this effect was largely limited to those genes identified as activated by Otx2 through our siRNA analysis.
Moreover using shRNA library screening several kinases, including the EGFR and HER2, were identified as activated in TNBC [ 19].
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