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We identified a restriction site that was present only in this sequence and digested the DNA (purified PCR product) with the HindIII restriction enzyme (A-AGCTT).
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The restriction fragment length polymorphism technique that we used identifies a restriction site not found in the CYP2D6*4 allele but that is present in other CYP2D6 alleles.
Last week's announcement that researchers had identified a new restriction factor that inhibits an early step of the H.I.V.-1 life cycle in immune cells is greatly encouraging as well.
We identified a NlaIV restriction site unique for the mRap1A cDNA and used it to distinguish between transcripts of mRap1A-retro1, -retro2 and that of mRap1A.
To determine the OspA type of the Borrelia spp. found in the joint aspirates, we performed another previously described nested PCR targeting the ospA gene (10 ), then sequenced and identified a virtual restriction fragment polymorphism by using CLC Main Workbench version 7.0 (CLC bio, Aarhus, Denmark).
Southern analysis using a human progesterone receptor (hPR) cDNA probe identified a germline TaqI restriction fragment length polymorphism (RFLP) defined by two alleles: T1, represented by a 2.7 kb fragment; and T2, represented by a 1.9 kb fragment and characterised by an additional TaqI restriction site with respect to T1.
Thus, we identified a putative DNA restriction-modification system.
The pcDNA-CMV-Lentivector 4-1BB shRNA expression plasmids were identified using a restriction endonuclease digestion.
Terms in these subsets are identified by a "Restriction" comment in the UniProt-developed GO browser QuickGO [ 20] alerting curators not to use them.
More than 150 tissue-specific differentially methylated regions (TDMs) have been identified via a Restriction landmark genomic scanning (RLGS) assay and expression of genes associated with these regions have been shown to be correlated with DNA methylation [ 12, 13].
Even though the first problem is nonconvex, we identify a suitable restriction of its feasible set that makes it convex while maintaining the same optimal value.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com