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To make sure that the correct clone was identified, a hand-held plate gridding/replicating device was used to stamp two nylon filters with the clones in the 384-well plate believed to contain the clone of interest.
The following steps are applied both off-line to build the reference database, and on-line to identify a hand posture.
Although we did not confirm H1N1 infection by viral culture or PCR, we preliminarily identified a relationship between hand washing behaviors and flu-like symptoms in men.
In silico analysis of the Sec62 sequence (http://www.bioinformatics.org/calpred/ index.html) identified a potential EF hand in the C-terminal domain of vertebrate Sec62, which may explain this Ca2+ effect (amino acid residues 308 319, see below).
This study reported a bacteriological survey of post-surgical eye infections in Nigeria; like this study, it also identified a lack of hand-drying facilities as an issue [11].
One recent study has identified a novel EF-hand-containing protein CRACR2A (CRAC regulator 2A), which binds to both STIM1 and Orai1 to stabilize their interaction, thereby enhancing SOCE in T-cells [ 40].
Molded-glass imitations can be identified with a hand lens, because the edges between the facets are rounded whereas cut glass has sharp edges.
Additional references were identified by a hand search.
A lid speculum was inserted and the ciliary body was identified using a hand held transilluminator.
Three additional trials were identified by a hand search of the bibliography of the remaining articles.
Additional references were identified by a hand search of original papers or reviews.
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