This heterogeneity has likely contributed to the high false positive rate of protein identification as described by Adamski, especially for protein identifications based on one peptide.
In case of protein identifications with mascot score values between 50 and 60 or protein identifications based on one peptide, individual peptide MS/MS spectra were checked manually and either interpreted as valid identifications or discarded.
These tools use the expectation maximization algorithm to separate correct from incorrect identifications based on a limited set of rules (one of the dominant properties, for instance, is the tryptic correctness of the peptide termini).
These MSAlign files were then subsequently searched with the E. coli BL21 database generated from NCBI (accession: PRJEA161949, containing 4192 protein sequences) with the alignment-based MS-Align+ algorithm for protein identifications based on protein-spectrum matches.
Two species of Ceriodaphnia have previously been reported from Churchill from the rock bluffs alone, but after extensive sampling our barcode results show only one species, suggesting the possibility that previous identifications based on morphology in this genus may be incorrect.
These identifications based on polychaete hard part anatomy were entirely consistent with the DNA-based identifications.
The city's chief medical examiner, Dr. Charles S. Hirsch, said the city had just begun to make identifications based on the first returns of analyzed DNA.
It raised questions about public/private dichotomies, art/design boundaries, and identifications based on gender, and recast the artist as a kind of art director and stage designer.
DNA sequences of trnL and rbcL genes amplified from ancient DNA extracted from intestinal samples confirmed and supplemented plant identifications based on microfossils and macro-remains.
Now the Washington Times reports that even those who are supposed to be protecting fliers from terrorists are being caught in the no-fly list's undiscriminating net".False identifications based on a terrorist no-fly list have for years prevented some federal air marshals from boarding flights they are assigned to protect," the Times informs us.
Mascot is used for identifications based on serial MS/MS datasets (data dependent acquisition), whereas IdentityE is used for identifications based on multiplexed MS/MS datasets (MSE or Hi-Lo Switching).
