Exact(1)
Ami, Vip, and InlA are well known virulence factors that contribute to listerial adhesion to and invasion of eukaryotic cells, and there identification validates the screening procedure.
Similar(59)
A straightforward approach to the treatment of protein identifications attributed to single peptide hits allowed the retention of potentially important low abundance identifications (validated by a cross-sample match and de novo interpretation of relevant spectra) while still eliminating potentially spurious identifications (global single peptide hits with no cross-sample match).
One compound TALA with the highest affinity was isolated for further in vitro pharmacodynamic validation and target identification to validate the screen results.
Campillos et al. [16] used adverse drug reactions profiles to develop a target identification model validated experimentally.
The developed automated system for material model parameter identification is validated by comparing the calculated with experimental response of the material.
Peptide/protein identification was validated by Peptide/ProteinProphet software tools [20], [21].
ProteinProphet [75] a program that applies a statistical model to infer protein groups from peptide identifications and validates these groups with a probability assignment, was also utilized.
These findings are illustrative of the need of precise species identification for validating biomedical research.
Theme identification was validated among researchers and discrepancies were resolved through discussions until concordance was achieved.
Protein identification was validated when at least three unique peptides originating from one protein showed significant identification Mascot scores (P <0.05) with False Discovery Rate FDRR < 5%) automatically calculated by Proteome Discoverer.
Further investigation using a fluorescent coumarin tagged bromophycolide A for sub-cellular localization and molecular target identification studies validated that heme crystallization was disrupted by the natural product bromophycolide A [ 31].
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