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Amplified products were run on 2% agarose gel for identification; samples with bright main strips between 400 and 450 bp were chosen for further experiments.
For genotype identification, samples were subjected to a previously described nested PCR targeting the 5S-23S intergenic spacer region (8 ).
For taxanes identification, samples were analysed by an Agilent 1100 HPLC system coupled to a 1100 MSD Ion Trap mass spectrometer, equipped with an electrospray ion source.
Prior to identification, samples were purified with C18-ZipTips (Millipore Corporation), eluting with 70% acetonitrile in 50 mM ammonium bicarbonate, and dried by vacuum centrifugation.
For Mφ identification, samples were incubated with rabbit anti-CD14 polyclonal antibodies at 2 μg/ml (Abcam) or biotinylated monoclonal anti-human CD80 at 2.5 μg/ml (Ancell Corporation) for 60 min at room temperature.
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A model of quarry identification, sampling, and characterization is drawn from work on Rapa Nui (Easter Island), and the fundamental precepts are outlined for future sampling experiments and provenance work in stone quarries generally.
A feedback control approach was used from the beginning of the sensor design in order to reach a good trade-off between circuit complexity and control requirements (inputs/outputs required for identification, sampling frequency, controller order, …).
For bacterial identification, sample water was pipetted out using micropipette and serial diluted until 10−5 for bacteria analysis.
Fishes, invertebrates, reptiles and marine algae will be the major groups encountered and snorkeling will be used for observation and collection (SCUBA certification is not required). Field work will be the main activity and attention will be given to collection methodology, identification, sampling techniques, research design and other useful field skills.
M.B. was responsible for the identification, sampling and processing of plant material and the co-supervision of plant-related work.
Beyond facilitating relatively simple tasks such as outlier identification, sample networks provide a novel perspective on more complex challenges such as group comparisons.
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CEO of Professional Science Editing for Scientists @ prosciediting.com