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The identification of the sequence of elementary steps in the reaction has been the subject of many investigations and has been corroborated through the application of formal Langmuir Hinshelwood (LH) and Eley Rideal (ER) mechanistic formulations [4, 5, 6, 7, 8, 9, 10, 11].
In this report, we confirm that the prrF1 and prrF2 genes encode PrrF RNAs of similar sequence and size, and we describe our identification of the sequence encoding a longer heme-regulated RNA, here named PrrH.
However, the subsequent identification of the sequence reads would require either the pooling of 600 PCR products targeting unique genetic regions, or, if multiple homologous PCR products were to be co-sequenced (i.e. multiple different products amplified using a single identical primer pair), an a prior knowledge about the exact sequence of each target.
Last column: SP identification of the sequence.
Direct Sanger sequencing allows the identification of the sequence alteration and is considered the gold standard.
Such differences emphasize the importance of the identification of the sequence of protein products to validate each gene annotation including its sequence coding area.
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After the identification of the sequences belonging to each individual and before the alignment, we removed the first five bases of every sequence corresponding to each of the barcodes.
Serotype identification of the sequences was achieved by comparison with Genbank data using the Basic Local Alignment Search Tool BLASTT) [ 42].
This reduced database is obtained from a 90% identity clustered version of the SILVA [ 18] database and is used to increase the rate of identification of the sequences similar to specific rRNAs,thereby reducing the computing time.
To facilitate species identification of the unidentified sequences, evolutionary relationships among the sequences were investigated using phylogenetic analysis [ 50].
c MS/MS data and procedure followed for the identification of the peptide sequence for the peptide from 32.26 min (red box in a).
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