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Identification of recombinants is done according to the guidelines defined in this paper.
In Sliding MinPD the identification of recombinants, ancestors and breakpoints is automated with no need for user intervention.
Identification of recombinants was performed by using the Recombinant Identification Program (RIP, http://hivweb.lanl.gov/RIP/RIPsubmit.html) (19 ).
The identification of recombinants was performed by using the Recombination Detection Program (http://darwin.uvigo.es/rdp/rdp.html) [ 16].
To simplify the identification of recombinants containing the suppressor alleles, we first introgressed mbk-2 (dd5 ) into the polymorphic Hawaiian strain to create mbk-2 (dd5 ) Hw.
For identification of recombinants, I- SceI was induced by 0.1% galactose for 40 min and Lys+ recombinants were selected by plating on SC-lysine medium.
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This program implements several methods for the identification of recombinant sequences and recombination breakpoints.
Recombination suppression inhibits accurate QTL mapping, positional gene cloning, MAS, and identification of recombinant genotypes with favorable combinations of alleles that are originally linked in repulsion.
The new ClosTron plasmids also offer blue white screening and other options for identification of recombinant plasmids.
Most available vectors used in C. glutamicum carry antibiotic resistant genes as a genetic labeling for rapid identification of recombinant strains, and antibiotics have to be added to maintain the vector when growing the cells.
Such approaches are an alternative for optimising resources in molecular biology research since the currently available commercial PCR-DNA cloning kits are expensive and involve using of additional reagents for the growth and identification of recombinant colonies (Cheong et al. 2009, 2012).
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