Here we review recent research on nanoparticle physicochemical properties important for protein binding, techniques for isolation and identification of nanoparticle-bound proteins, and how these proteins can influence particle biodistribution and biocompatibility.
The known excitation spectra for silver nanoparticles were used for specific identification of the nanoparticles using UV Vis spectrophotometer.
The current study developed a rationalized method for the quantification and identification of engineered nanoparticles (ENPs) in wastewaters.
Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) allowed the identification of spherical nanoparticles with about 150 nm in average diameter.
Identification of Fe2O3 nanoparticles inside the cells was straightforward because the particles maintained their faceted feature.
However, the pulse test setup allowed for identification of possible nanoparticle-specific effects of CuO nanoparticles, since more pronounced reproductive effects were found for CuO nanoparticles than for CuCl2 when the exposure concentration was expressed in terms of the measured dissolved Cu.
Varying the ratios of PAT-SPN and FA-SPN enables the identification of a nanoparticle composition optimized for in vivo delivery (effectively shielded zeta potential) that achieves a high level of specific uptake by cancer cells (where there is colocalized presence of MMP7 activation and FA binding).
Calzolai, L., Franchini, F., Gilliland, D. & Rossi, F. Protein-nanoparticle interaction: Identification of the ubiquitin-gold nanoparticle interaction site.
To make a precise identification of the presence of nanoparticles by light microscopy, their presence was only considered as proven when the three microscopies employed (phase contrast, bright field and dark field) provided correlated images (see additional files 1, 2, 3: correlative images 1, 2, 3 for the micrographs used to select the nanoparticle-containing cells to be analyzed at TEM).
