SN performed isolation and identification of isolates, molecular typing and susceptibility testing, analyzed the data and drafted the manuscript.
Complete concordance was seen between the biochemical identification of isolates and the N-PCR identification of mycobacterial species prior to isolation.
Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks.
Molecular typing methods have permitted investigation of the NTM transmission patterns21 discrimination between drug resistant and drug susceptible isolates22 and identification of isolates with high virulence capacities23.
Concordance in the identification of isolates by the visual, CM-PCR assay, and biochemical identification was seen.
In addition, applications of the fluorescently labelled probes for in situ identification of isolates and for the in situ localisation of A. brasilense on maize roots were demonstrated using confocal laser scanning microscopy.
Quality control during mass production of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), and studies on environmental fate following the use of this virus as a biological pesticide, would be facilitated by a rapid method for the detection and identification of isolates.
In this study we compared the performance of the Biolog GEN III identification system (hereafter, GEN III) with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing in the identification of isolates of veterinary interest.
Tuf-PCR was used for identification of isolates belonging to genus Enterococcus according to protocol Ke et al. (1999).
Table 2 Identification of isolates from the genus Pseudomonas by partial gyrB sequencing Species Query cover Identity Isolate 8.1 P. syringae pv.
Biochemical studies were carried out for the identification of isolates (F1 and G2) as follows: Catalase test, Methyl-Red Test, Voges Proskauer Test, Citrate Utilization Test, Phenylalanine Deamination Test, Urea Hydrolysis Test, Starch Hydrolysis Test, Casein Hydrolysis Test, Triple Sugar Iron (TSI) test, catalase, oxidase, urease and sugar fermentation tests.
