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To define whether these potential OTEs could influence the identification of interacting genes, we generated a list of interacting dsRNAs, their primary intended targets and their potential off-targets as predicted at the 19-nt level by NEXT-RNAi [ 11].
This technique, entitled "two-hybrid" or "interaction trap", enables not only the identification of interacting partners but also the characterization of known interaction couples and even embodies the technological means to manipulate protein-protein interactions.
Thus, several computational approaches have been developed to predict protein-protein interactions utilizing existing bioinformatics data such as gene proximity information [ 18, 19], gene fusion events [ 20, 21], gene co-expression data [ 22- 24], phylogenetic profiling [ 25], orthologous protein interactions [ 26] and identification of interacting protein domains [ 27- 30].
Liu, Q. Z. J., Wang, H. & Zhuang, M. Identification of interacting proteins using PUP-IT.
Comments on: the identification of interacting networks in the brain using fMRI: model selection, causality and deconvolution.
A systematic identification of interacting compounds may provide a means to purify more proteins, as well as a method to ascribe function to new proteins.
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As this method is based on the unbiased identification of sequences interacting with specific baits, there are unique opportunities for unravelling the secrets of how the genome functions in 3D.
Therefore, myc tagged PrPSc may also allow for identification of proteins interacting with PrPSc.
Therefore, the 3'terminal parts of these 3'UTRs seemed to be good candidates for identification of proteins interacting with the motif clusters.
Identification of proteins interacting with mutant p53 (mutproteinotein in tumors from Trp53 R172H/R172H mice.
(A ) Work flow for identification of proteins interacting with mutp53 protein.
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