Sentence examples for identification of binders from inspiring English sources
For identification of binders, supernatants containing monoclonal scFv were incubated in the antigen coated plates for 1.5 h at RT followed by three PBST washing cycles.
Moreover, CCCP, as a more objective method, enables the identification of binders of biochemical classes that were previously unexpected [31]– [33], including those without enzymatic function.
As the paint used in sculpture requires an organic binder to fix the pigments to the support, the lack of identification of organic binders has to be ascribed to the use of a fresco painting technique, and may instead be taken as proof of the difficulty to characterize ancient paint media.
Thus, our approach led to the identification of potent binders of the Dvl PDZ domain and the findings provide novel insights into structure-based approaches to design high-affinity binders for the Dvl PDZ domain.
For the identification of monoclonal binders, colonies from the titre plates of the eluted phage particles after panning were picked and soluble scFvs were produced in microtitre plates as described before [105].
The identification of monoclonal binders was performed as described before [ 43].
The affinity is linked in the same figure to the EIC for identification of the binders.
The availability of the genomic sequence [ 26] provides new horizons in analyzing the potential immunome of the bacilli, using in silico identification of CTL binders.
Furthermore, co-crystallization screening allows the identification of weak binders, such as fostamatinib (provided they are soluble at 1 mM in 10% DMSO), which are otherwise typically discarded during HTS campaigns or remain undetected due to limited assay sensitivity.
To explore the fact that we could have been losing 60-80% of total scFvs due to denaturation in our antibody microarrays [ 5, 53], we decided to test all the scFvs by ELISA to compare the performance of the microarray and verify the usefulness of antibody microarrays for identification of scFv binders.
The identification of monoclonal binders was performed as described in [ 69] with the following modifications: 96-well polypropylene (PP) micro titer plates (Greiner, Germany) containing 150 μL 2xYT-G/A (2xYT [ 67] supplemented with 100 mM glucose and 100 μg/mL ampicillin) were inoculated with colonies from the titration plate of the third panning round.
