Officials at Parsons Infrastructure and Technology Group, which runs the state inspection program, told the newspaper that employees were trying to bypass a fault in the computer system that caused it to have problems reading the cards and forced employees to enter the 17-digit vehicle identification number manually.
Metabolite-features were quantified after mass spectral deconvolution (ChromaTOF software 1.00, Pegasus driver 1.61, LECO), and their chemical identification was manually assessed using the NIST05 software (http://www.nist.gov/srd/mslist.html) and the mass spectral and retention time index collection of the Golm Metabolome Database [48].
Protein spots selected for identification were manually excised from the gels, and subject to trypsin automated in-gel digestion in a MassPrep Workstation (Micromass, Manchester UK) [ 63].
All identifications were manually validated and the proteins were selected and validated on basis of MOWSE scores, peptides matches, and % sequence coverage.
With the action word set, transcription factor, and gene names identification, we then manually establish several patterns that can describe the transcription factor and target gene regulation relationship from the answer sets to be our seed patterns.
Each protein identification was verified manually.
22 Identification was confirmed manually using the test of carbohydrate assimilation.
To increase the sensitivity of immune gene identification we used manually curated opossum (Monodelphis domestica) immune gene lists [ 36, 37] of 1,549 opossum genes.
Cell identification was done manually using a graphical cluster cutting tool, with 2D projections of the multidimensional parameter space consisting of waveform amplitudes.
For protein identification, spots were manually cut out from SDS-gels, transferred to 96-well microtiterplates (MTP) and processed on an automatic Ettan TA Digester (GE Healthcare, Freiburg, Germany).
