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HCDiff identification by the Reference Mapper software is based on a combination of flow signal and quality score and requires a minimum number of sequences, the presence of both forward and reverse reads covering the difference etc.
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The identification assigned by the reference laboratory was used for final analysis.
The latter case is illustrated by the identification of the reference standard chromatographic peak and FTIR spectrum of the potent psychotropic amphetamine derivative N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine N-methyl-1- 3,4-methylenedioxyphenyl -2-butanamine
In the design three parameters based on covariance Pk and Qk are calculated The stochastic variable depends on the three gains and three functional estimation errors, respectively, developing the stochastic identification by a reference model that converges in almost all points with 10 iterations in recursive estimation.
The reference strains of all species analyzed showed unique species‐specific ITS patterns which were further present in 49 clinical isolates of [P.] pneumotropica biotypes Jawetz and Heyl and [A.] muris allowing their identification by comparison to the reference strains pattern.
For the assembly of the document, reviews and randomised controlled trials in adults were identified from Medline, Embase and the Cochrane Database, as well as from reviews on ADHD in children and cross-referencing and identification by the participating experts.
For our identifications we solely used the high confidence differences (HCDiffs) extracted by the Reference Mapper software (Roche).
The best matches in GenBank/UNITE of all unique sequences are listed in Additional file 2 and a list of all detected OTUs is presented in Additional file 3. Based on identification by most similar reference sequences, 68.8% of the 576 sequences belonged to Basidiomycota, 30.7% to Ascomycota, 0.35% (two sequences) to Zygomycota, and 0.17% (one sequence) to Glomeromycota.
Furthermore, identification by reference spectra libraries does not succeed in combination with contaminated and / or degraded micro plastics.
A further improvement of the FTIR identification system was achieved by combining the reference database and the ANN.
It shows better performance than several available module identification tools by testing on the reference responsive gene sets.
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