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For protein identification, approximately 0.8 µl of the peptide mixture was spotted onto a target plate and covered with the same volume of matrix solution (α-cyano-4-hydroxy cinnamic acid (Sigma), 8 mg/ml in 70% (v/v) acetonitrile/1% (v/v) formic acid) and allowed to air dry.
Results show less than one third of case-patients (28.3% and 31.1%) were registered as contacts before case identification; approximately two thirds (61.1% and 67.7%) had no registered contacts.
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Approximately 1,000 proteins were detected in each small sample run followed by the identification of approximately 4,000 possible expressed protein targets.
Approximately 250 of the protein spots were analyzed by mass spectrometry, resulting in the identification of approximately 180 unique proteins.
This resulted in the identification of approximately 20 million SNPs.
Although this approach led to the identification of approximately 1800 proteins, no cytokine or chemokine proteins were identified except for acute phase proteins.
The R. solanacearum genome sequence recently allowed the identification of approximately 80 putative effectors whose targets in the plant cell and their roles during infection remain to be elucidated [14], [15], [16].
A comprehensive analysis of samples led to the identification of approximately one hundred proteins, more than thirty of which were observed with iTRAQ signature mass intensity ratios that suggested specific co-enrichment with members of the prion protein family (Figure S1, Watts, J.C., et al., in revision).
For the microarray, the turnaround time from positive blood culture bottle to species identification was approximately four hours.
In total 92 cDNA-AFLP primer combinations were performed and this screen lead to the identification of approximately 7300 independent transcript-derived fragments (TDFs) of 100-500 bp.
More than 180 million purity filtered reads were used for de-novo assembly resulting in identification of approximately 97000 unique transcripts.
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