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In the adult the number of both motor target neurons and their modulatory neurons is restricted to tens of identifiable cells.
Among the identifiable cells in the abdominal ganglion of Aplysia californica are five that generate bursting rhythms endogenous to the cells.
Using a wide spectrum of different fluorescent markers, including drug-selectable enhanced green fluorescent protein (eGFP)- and dTomato-blasticidin-S resistance fusion proteins, LeGO vectors allow simultaneous analysis of multiple genes and shRNAs of interest within single, easily identifiable cells.
Cell survival after any treatment was assessed by counting clearly identifiable cells with intact morphology, whereas dead cells atrophied and degenerated.
To visualize synapses in vivo in genetically identifiable cells, we explored strategies to label active zones, sites of presynaptic neurotransmitter release (Zhai and Bellen, 2004).
In these animals, numerous SL-ir neurons are evenly distributed along the two ventral medullary cords, with no indications of segmental arrays, let alone stereotypic individually identifiable cells.
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Most of us had a swab sample taken on arrest and our identifiable cell clusters have languished on police files even if charges were dropped or we were found not guilty in court.
As none of these proteins contains any of the identifiable cell-binding peptide motifs, such as the arginine-glycine-aspartic acid (RGD) sequence, the process of cell attachment has been suggested to be non-specific, likely based on electrostatic interactions (Minoura et al. 1995b).
Unlike the authors, I thus definitely think that LUCA has been an identifiable cell line.
When clearly identifiable cell clones had formed, the colonies were fixed with methanol and stained with 0.5 % crystal violet.
The assay is not only amenable to additional tau phosphorylation sites, but also to those identifiable cell-based endpoints related to other neurodegenerative diseases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com