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Regarding the direction of gene expression regulation, 1968 probes were identically regulated while 150 were oppositely changed during C-VSMC and osteoblast development (Additional file 2: Table S1).
Regarding the comparative transcription profiling, we identified 57 ECM genes identically regulated by C-VSMCs and osteoblasts.
This is not a serious drawback since all previous evidence suggests that these two duplicated genes are identically regulated, but the caveat must be added that we could not tell them apart.
To identify whether only specific groups of genes were identically regulated by C-VSMCs and osteoblasts, we have selected differentially expressed genes during VSMC development into C-VSMCs and during MSC differentiation into osteoblasts.
C = control strain; E = expressing strain; 8 = hypoxia; 11 = oxygen limitation; 21 = normoxia As illustrated in the Venn diagrams, only around fifty percent of these genes were identically regulated in both the Fab-expressing and the control strain, indicating a different behaviour under hypoxic conditions.
To test the importance of co-transcription, we carried out Lyt and CII protein production in separate reactions in the simulation, as if they were transcribed from distinct, though identically regulated promoters (p.lyt/p.cII).
Similar(54)
The industries argued that the facilities have different purposes and operating conditions and shouldn't be regulated identically.
However, genes carried on plasmids may not be regulated identically to genes at their native locus.
One such case is that all steps in a metabolic pathway are regulated identically, i.e. their activities being modulated by the same factor.
Despite the fact that C-VSMCs had an overall transcription profile distinct from osteoblasts, the two cell types regulated identically subsets of ECM and biomineralization genes.
In contrast to p53, genes regulated by NFκB responded almost identically in irradiated and bystander cells.
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