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Mice were treated with 50 mg/kg BBG i.p. or an identical volume of saline 30 min before NTG treatment.
This increase was significant at all levels of the TNC when compared to control animals (white bars), injected with an identical volume of vehicle.
Wild-type mice were treated with 600 μg/kg sumatriptan/50 mg/kg BBG i.p. or an identical volume of saline 5 min after NTG treatment.
In P2X7+/+ mice, treated with sumatriptan 5 min after NTG, PWT was higher than in mice treated with an identical volume of saline (at 1 h: 98.74 ± 1.66%, n = 10; 93.43 ± 1.80%, n = 10, p = 0.0375 in NTG + sumatriptan and NTG + saline treated mice, respectively, Figure 2).
In both groups, the infusion was either an initial 1 mg/kg cisplatinum and 2 mg/kg adriamycin followed by a second infusion of 1 mg/kg cisplatinum and 4 mg/kg adriamycin or an identical volume of normal saline at identical points (Fig. 1).
Intracisternal injection of 10-fold concentrated IS (10 mM histamine, serotonin; 1 mM bradykinin, prostaglandin E2; pH 5.5; adapted from [35]) resulted in an increased expression of c-fos within the TNC as compared to controls, which were exposed to an identical volume of vehicle for the same duration (Fig. 1a).
Similar(28)
The blank was prepared in the same way as the soil samples using identical volumes of reagents.
As controls, identical volumes of 20% de DMSO (without PMA) were added to 500 μl of heat-killed and live cells.
Identical volumes of 5.10−3M silver nitrate (0.35 mL) and 10−2M NaBH4 (0.15 mL) were quickly added simultaneously to each pill box under vigorous stirring.
Although every H2O molecule is identical, volumes of water aren't delivered at equal cost.
Age-matched control animals were inoculated with identical volumes of sterile Brucella broth alone.
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