The kinetic profiles of both isozymes were nearly identical: maximal turnover rates, reflected by the kcat values, were (1.8 ± 0.3) × 10 3 s–1 for PHLPP1 and (1.8 ± 0.1) × 10 3 s–1 for PHLPP2.
The assays were identical to the multiple turnover reactions, except for the amount of ligase was increased to 13.8 pmol.
This quality control can be represented through the degradation rates assigned to each mtDNA species, which may differ (for selective quality control) or be identical (for non-selective turnover).
To deal with rising levels of O2, a direct strategy is to reduce it by four electrons all the way to water; this process may have been catalyzed early on by the widespread cytoplasmic flavodiiron proteins, which perform this reaction with turnovers almost identical to those of the "canonical" membrane attached heme copper or cytochrome bd oxygen reductases.
Applying the same assay to CPY*-D1 and CPY*-D2, their turnover profile is identical to CPY*.
TSET is non-essential in Dictyostelium, but may act in plasma membrane turnover, and is essentially identical to the recently described TPLATE complex, TPC.
These kinetic parameters are almost identical to those observed in the multiple-turnover experiments, suggesting that the same step is rate-limiting for single-turnover and multiple-turnover conditions.
The turnover rate constant, c, was nearly identical in DMSO, AZD8055 and rapamycin-treated cells, suggesting that AV degradation was unperturbed by AZD8055 or rapamycin treatment.
