A follow-up study with an identical set-up that introduces stress during development should give a more detailed insight into the role of development in the regulation of lifespan.
After 1 and 7 h of bioreactor supernatant perfusion, including activated CYCL, the ZR 751 cell line showed an EAR of 98.99%±3.15 (mean±SD) and 81.32%±10.18 (P<0.05), respectively, as compared to controls (bioreactor supernatant from the identical set-up without CYCL).
Using the identical set-up as described before (Baumann et al., 2014), we determined a half time of recovery (t1/2) of 4.2 min for wild-type hyphae.
We performed two independent experiments of identical set-up with the antibiotic pairs CIP/DOR and GE N/CEF(experiments 1 and 2, respectively) and, in each case, a total of eleven treatment protocols that belonged to four treatment types: regular and random sequential treatments (2× four protocols), monotherapy (two protocols) and a no-drug control (see below).
To examine the effect of a high number of cycles in MSP, a small interlaboratory study was performed between The Canisius Wilhelmina Hospital in Nijmegen, the Netherlands (CP, ET) and the Fondazione IRCCS Istituto Nazionale Tumouri in Milan, Italy (GZ) with identical set-up (DNA samples, primers and protocols) (Field et al, 2009).
When Cleveland State University researchers came up with two fictional job candidates, both with identical credentials, they set up a very important but subtle distinction between the two of them.
— arabist (@arabist) 3 Apr 13 The sudden disappearance of the official feed also led, inevitably, to a parody account with an almost identical handle being set up to fill the void.
From each patient two sets of identical cultures were set up in 96 well plates (6 wells in each set).
Identical reactions were set up without the addition of reverse transcriptase to test for the presence of contaminating genomic DNA using primers to C. parvum rRNA and 50 cycles of PCR under standard conditions.
In parallel, identical experiments were set up, except that the diluted plasma samples were preincubated with either 20 nM MD, 20 nM MDTC or 10 nM MDTCS for 30 min, before applying to wells coated with full-length ADAMTS13.
