Surprisingly, identical gene quantification results were obtained for only 16.3% (about one sixth) of genes.
We showed that the spheroids cultured in NCP could be similarly assayed using an identical ATP quantification method.
As shown in Figure 6d, the expression profiles of Pu in response to both xylene species were virtually identical, as quantification of the signal intensities in both conditions gave nearly overlapping induction curves.
An aliquot of B. cereus spores was resuspended under conditions identical for the quantification of alanine in the supernatant (see above).
For quantification, identical samples were processed for flow cytometry, with and without acid stripping.
If this prerequisite is met, ablation, transport, atomization, and ionization of sample and standard can be considered to be (nearly) identical, enabling reliable quantification.
Gene Chip Operating Software (GCOS) was used for calculation of detection calls, signal values and for calculation of the target intensity scaling of each array to an identical value and quantification of the signal log ratio.
In support of the quantitative performance of our miR-Seq, the differential expression of all three miRNAs measured by qPCR was nearly identical to the quantification by our miR-Seq.
This approach may be desirable on a single-site basis, but for the purpose of this work (as a precursor to a multicentre evaluation) it was not an ideal method due to the need for additional experimental work and the inability for all sites to have an identical approach to quantification.
As a normal control was performed with each experiment, the exposure time may have varied between experiments due to changes in the signal intensity; however, within each experiment the exposure time for each fluorochrome was identical so that quantification of the signal was applicable.
