Four additional control experiments were run with identical methods as used for the Direction-Training Group with the exception as stated here (see Figure 1).
PB MNCs and total RNA were isolated from the blood using the identical methods as described for collection of murine cells and RNA.
The partitioned matrix was analyzed using identical methods as the full plastome analysis.
For controls, we included data on healthy blood donors from our initial study, which used identical methods as the present one [ 17].
We constructed a T7 phage-displayed 12-mer peptide library randomized using codon-corrected trinucleotide cassettes (T7 Trinuc) with identical methods as previously described [ 9].
The diverse non-muscle cell cultures, which included fibroblasts, melanocytes, hepatocytes, and astrocytes, were profiled using the same type of exon microarrays and identical methods as for the myoblasts and myotubes.
Moreover, no fluorescent eGFP-degradation products show up on native gels, as found previously in [30] by an identical method.
As a comparison, a home-made jujube extract (J2) was also prepared in our laboratory by an identical method.
As far as possible, we sampled from the same patient population and used identical methods to the original study so as to minimise any sources of variation other than the database itself.
Using identical methods, we also identified Sk27 as a type 2F strain (Table 2).
