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And there is a costly trade-off: DioLight bulbs give off around 30percentt less light than standard bulbs of the same wattage, so to get identical brightness, you have to use a higher-wattage bulb that gobbles up more electricity.
Image processing was performed using Adobe Photoshop CS2 (Adobe Systems Incorporated), but maintaining identical brightness and contrast settings for images of infected cells and controls.
Images acquired on different channels for common samples were processed with identical brightness and contrast settings.
Final images of either CEPs and ADEs or PDEs were processed using identical brightness and contrast values.
Overlay of the images and adjustment to identical brightness and contrast settings was performed in ImageJ and Adobe Photoshop.
Z-stacks were reduced to the composite image only for the presentation purposes, by projecting individual layers, with centromeres in focus, onto a common plane and the identical brightness range was kept for all comparable panels of any given Figure.
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Colour vision test For colour trials, 100 unique pairs of shapes (Fig. 1 c, d) were filled with the green and the blue that were identical in brightness ("Matching brightness of colour" section).
Identical exposure and brightness level settings were used for test and control samples.
1 μm thick serial images (25 μm in total) were captured with a 63x objective at zoom 2 in 3 randomly chosen areas of the ring, at identical conditions of brightness, contrast, and laser power for all of the experimental groups.
1 μm thick serial images (25 μm in total) were captured with a 63x objective at zoom 2 in 3 randomly chosen areas of the ring, at identical conditions of brightness, contrast, and laser power for all of the experimental groups with the 488 nm/590–620 nm line of the microscope.
They have similar resolution; viewing angles are almost identical; and even max brightness and outdoor visibility look even.
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CEO of Professional Science Editing for Scientists @ prosciediting.com