In contrast, all of the genes shared between the two isolates are at least 99% identical over 99% or more of their length, as determined by blastn.
We used the murine intranasal model of cryptococcosis to compare the lethality of clinical and environmental strains of serotype A that possessed identical genotypes as determined by amplified fragment length polymorphisms (AFLP) and multilocus sequence typing (MLST).
Perform additional reconstructions, using list-mode data and identical settings as determined at step 3, for shorter scan times at 75, 50, 25, 12.5, 6.25 and 3.13 % of the original scan duration of 10 min.
Fortunately, RPB1 mRNAs generated from all four alleles contain identical 3'UTRs, as determined by analysis of RPB1 mRNA by 3' RACE-PCR (data not shown).
Given the lack of within-group differences for RAG 1A, genes RAG 1 B, GHR A and B were sampled in a subset of specimens, with identical results, as determined by comparing recovered sequences to those for R. rattus in GenBank and by performing relevant phylogenetic analysis (Fig. 2).
This data processing resulted in the identical SNPs as determined by comparing the WT with one of the mutants.
The instrument was flushed with nitrogen at a flow rate of 5 L per min. For recording of far UV spectra (180 260 nm), recombinant proteins dissolved in 5 mM sodium phosphate buffer (pH 7.0) were brought to identical concentrations as determined by measuring the optical density of the solutions at 280 nm.
As in controls, plasma cells in anti-BAFF-R treated mice were localized in the red pulp and were identical in numbers, as determined by ELISpot assay (data not shown).
Briefly, Caspase-Glo 3/7 reagents were reconstituted and added to identical cell numbers (as determined via flow cytometry) and volumes for the assays (H9, LACZ, 133, 826), negative control samples and blanks.
