As expected, when we permeabilized cells with Triton X-100 (ideal for visualizing actin, [17]), cofilin rods were not detected by rhodamine-phalloidin (Fig. 2c).
When cells were permeabilized with ice-cold methanol (which is ideal for visualizing cofilin rods, [3], [15]), we also found rod-like structures immunoreactive for cofilin in Tg19959 primary neurons, but only rarely in wildtype littermate neurons (Fig. 2a).
Today's molecular imaging possibilities are ideal for visualizing the pharmacokinetics of the effectors used in new treatment strategies such as gene therapy [ 1] and therapeutic vaccination [ 2].
It can be envisioned that such an approach would be also ideal to visualize and localize signaling events in response to other pathogenic bacteria.
Compared to these other techniques, synchrotron x-ray phase-contrast imaging [ 4, 5] is ideal for visualizing well-defined internal structures that have different mass densities.
However, it would be more ideal to visualize membrane proteins in live cell membranes at the single-molecule level, using HS-AFM.
The noninvasive nature and high axial (3-5 µm) and lateral (5-30 µm) resolution are ideal to visualize the underlying structures and detect changes in the retinal tissue of healthy and diseased eyes.
Clearly visualize your ideal image, style and desired characteristics and work towards those goals.
"I wanted to share with trans youth the power of visualizing an ideal image of themselves and give them the opportunity to create it," Rocero told The Huffington Post.
Because negative-stains are non-ideal for visualizing nucleic acids (Grob et al., 2012), we further assessed DNA binding by biotin-labeling the duplex end of the oligonucleotide, mixing the CMG DNA samples with streptavidin, and collecting new single-particle EM data.
