50, 51 Genome-wide cDNA microarray analysis of gene expression profiles in comprehensive tumor types allowed us to identify various novel TAAs that show unique and ideal gene expression patterns, such as CTA and OFA.
The data were normalised using the average expression intensity of all of the spots on the filter which gave results consistently centred on the ideal gene expression ratio of 1, indicating no change between duplicate samples.
The qa-2 promoter is useful, but not ideal, for gene expression manipulation because it is highly influenced by the nutritional state of the culture, and expression levels are generally not greatly enhanced.
Total RNA is ideal for gene expression profiling from clinical specimens, small amounts can be used because the methodology preserves the relative abundance of the different mRNAs in the original sample [ 4].
An ideal inducible gene expression system should have high inducing efficiency, stringency, and specificity to an exogenous inducer innocuous to the host cell, with which the expression level of the target gene as well as the function of the related pathway can be easily regulated theoretically [ 32].
Viral-mediated gene delivery has proven ideal for mosaic gene expression in vivo [15], [16] but can also be used in slice cultures [17], [18].
FHL1 interacts with components of the cytoskeleton [22] and with transcription factors and shuttles between the cytoplasm and the nucleus [23] making it an ideal regulator of gene expression in response to cytoskeletal changes.
Theoretically, the comparison of cells of the same lineage at a similar differentiation stage would be ideal for human gene expression analysis, however, in practical terms this was difficult to perform, since samples of both T and NK lineage were included in this study.
Together with simple nutrient demand, the potential to regenerate redox cofactors at a high rate (Blank et al. [2008]) and its amenability to genetic manipulation, P. putida is an ideal host for heterologous gene expression (Meijnen et al. [2008]).
Tetracycline is rapidly metabolized and cleared from the body, making it an ideal drug to control gene expression.
The 5S RNP is therefore an ideal marker to coordinate gene expression from all three RNA polymerases and the rate of ribosome biogenesis, itself an essential aspect of cellular growth.
