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Occupancy of glycosylation sites was quantified by comparison of XIC precursor ion intensities of deglycosylated [O]-labeled and nonglycosylated peptides acquired on the TripleTOF 5600 mass analyzer using an IDA workflow following PNGaseF deglycosylation under H2[O] as described.
Analysis used an Information Dependent Acquisition (IDA) workflow with one full scan (400 1600 m/ z) and 50 MS/MS fragmentations of major multiply charged precursor ions with rolling collision energy.
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Workflow is pretty handy.
Ida frequently rests: "When anybody needed Ida Ida was resting.
Beloved husband of Ida.
Adele and Ida arrive.
Ida Jolles, of Vienna.
Ida comes back safe.
Ida winced.
Ida wondered.
Ida thought.
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