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To design primers for our high multiplex amplicon barcoding protocol, we adopted the "Primer ID" design strategy [ 16] by inserting a molecular barcode region (random 6 to 12mer) between the 5′ universal sequence and 3′ target specific sequence in one of the two primers for each amplicon.
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it id designed to be usable by someone who does not even want the complexity of using a computer.
It is shown that an ID designed to resist high amplitude excitation is able to perform well at lower amplitude.
ID designed the protocol and analyses with input from the other authors.
ID designed the research project, collected material and wrote the manuscript.
NH and ID designed the broader study of bipolar disorder within which this protocol fell, recruited and evaluated subjects, and commented on the manuscript.
ID designed the study, participated in acquisition of the data, analysis, and interpreting the study results, revised the manuscript, and provided inputs in various drafts.
SM designed and carried out the computational experiments; PS developed the program and analyzed TRANSFAC PWMs, A Kel provided biological insight and actively participated in discussion of the project and writing the paper, A Kondrashov and ID designed and led the project.
We used the German version [ 18] of the Developmental Behavior Checklist (DBC) [ 19] to assess behavioral and emotional problems in the five mutation carriers with ID (designed for children aged 4 to 18).
Additionally, there is a general search engine that supports queries of gene name, symbol, pathway, GO term, and LocusLink ID designed for direct access to any gene detail page or pathway/GO detail page.
Many children with ASD are also diagnosed with Intellectual Disabilities (ID), yet design studies involving children with ASD and ID are scarce.
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