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Images were quantified using open-source ICY software plug-in Manual Counting55.
Evaluation of bacterial location was performed on three-dimensional z-stacks using ICY software.
Three-dimensional images of synoviocytes were rendered using ICY software (http://icy.bioimageanalysis.org) and the 3D Rotation plugin.
Fluorescent signals were quantified using a specific plugin of the Icy software (de Chaumont et al., 2012) (http://icy.bioimageanalysis.org).org
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Parasites and macrophages were enumerated in the max projections by the use of the Icy digital image analysis software [ 38].
At the same time as Mr Zuckerberg was giving himself a cold bath, Microsoft's current chief executive, Satya Nadella, was being doused with icy water by a group of software developers.
The images obtained were used in measurements of adipose tissue from each animal using the software ImageJ (version 1.32j, from NIH) and ICY (version 1.3.6.0, from http://www.bioimageanalysis.org/).org/
Icy streets.
Icy toes?
Not too icy.
"Icy fingers, grasping".
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