Frozen samples were transported to the International Centre for Genetic Engineering and Biotechnology (ICGEB) in New Delhi, and kept in liquid nitrogen until thawed for cytoadherence assays.
The authors are grateful to Professor Virander S. Chauhan, Director (ICGEB) for providing all the necessary facilities for conducting the experiments and critically reviewing the manuscript.
C57BL/6 and PD-1 deficient mice (obtained from Dr. Tasuku Honjo, Yoshida-konoe, Sakyo-ku, Kyoto University, Japan, via Dr. Megan Sikes, Transplantation Biology Research Center, Harvard Medical School, Boston, MA, USA) on a C57BL/6 background were bred in our specific pathogen-free animal facility at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
Funding for open access charge and for the services of a scientific writer/editor: ICGEB.
W2 strain was kindly provided by Dr. Pawan Malhotra (ICGEB, New Delhi).
This work was supported by internal grants from the International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
This work was supported by the Short-Term Fellowship (Ref. No. F/ROM11-02 F/ROM11-02warded through the International Center of Genetic Engineering and Biotechnology (ICGEB).
These EST sequences were made available by our collaborators in International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi and University of Hyderabad, Hyderabad, India.
Mice were housed and handled according to institutional guidelines, and experimental procedures approved by the International Centre for Genetic Engineering and Biotechnology (ICGEB) board.
Mice were housed and handled according to institutional guidelines, and experimental procedures approved by the International Centre for Genetic Engineering and Biotechnology (ICGEB) board, with full respect to the EU Directive 2010/63/EU for animal experimentation.
This work is based upon research supported by the International Centre for Genetic Engineering and Biotechnology (ICGEB), South African Research Chairs Initiative of the Department of Science and Technology and the National Research Foundation, the South African Medical Research Council and the University of Cape Town.
