Sentence examples for iced cold from inspiring English sources

To isolate protein extracts from primary hepatocytes, cells were washed twice with iced cold PBS, mixed with RIPA Lysis buffer, and then sonicated on ice for 20 s prior to collecting protein solution.

Set the bowl over another bowl of iced cold water to chill the soup quickly.

I find this easiest by swirling them in a deep bowl of iced cold water.

Drop the courgette shells into the water, return to a simmer, cook for two minutes, then drain and refresh in iced cold water.

A couple of times a year, I totally enjoy an iced cold Mexican Coca Cola from a glass bottle with a taco.

Hopefully we will see Coderre out there at 5 30 AM, enjoying an iced cold cruiser with the people of his city.

Briefly, after treatment as above, dIMR32 cells were harvested, cells were washed with PBS, centrifuged at 1000 rpm for 10 min, the supernatant was discarded and 200 μl of iced-cold 1xRIPA buffer was added to the cell pellet in order to lyse the cells for protein extraction.

The cells were centrifuged at 1250g for 5 min, and the pellet was lysed with iced-cold RIPA buffer (1% NP-40, 50 mM Tris-base, 0.1% SDS, 0.5% deoxycholic acid, 150 mM NaCl, pH 7.5), to which was added freshly prepared phenylmethylsulfonyl fluoride (10 mg/mL), leupeptin (17 mg/mL), and sodium orthovanadate (10 mg/mL).

I don't know whether they do anything with the data, but at least I can stop thinking about it as I munch on some tasty chips and sip my iced-cold Pepsi.

The cells were fixed in 70% iced-cold ethanol with vortexing and incubation for 1 hr at 20°C.

Cells were trypsinized, fixed in 70 % iced-cold ethanol, and stored at -20°C for 72 hours.