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Raji cells were incubated for 30 min on ice with different amounts of biotinylated p17 or p17Δ36, ranging from 50 to 400 ng/ml.
DCs were labeled with the cell markers CD11c and CD11b, and 5×105 or 1×106 stained cells were incubated on ice with different concentrations of unlabeled CPMV particles (from 0 to 100 µg of particles, where 1 µg of CPMV contains 1×1011 particles).
For the binding competition assay 1×106 or 5×105 DCs were stained with the above anti-CD11b and anti-CD11c antibodies and incubated overnight on ice with different amounts of unlabeled CPMV (0 µg, 1 µg, 10 µg, 100 µg, and 1000 µg).
Spleen cells from untreated WT mice, LPS treated WT mice, Neu1 knockout mice or culture cells were washed in flow staining buffer (1× PBS, 2% BSA), and incubated for 1 hr on ice with different directly conjugated-antibodies in flow staining buffer.
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This paper focuses on the control of marine riser of a position-moored drilling vessel operating under level ice regime with different ice thicknesses.
But when water is compressed to about 20,000 atmospheres and cooled, other varieties of ice form, with different molecular arrangements and linkages among hydrogen and oxygen atoms.
Fig. 7 CTP retrieval errors estimated by retrieval simulation for ice clouds with different CTTs.
(3) A pellet size regulator, which is used for extrusion of ice ribbon with different dimensions.
Changing the process parameters that influence the cold transmission efficiency from the bottom to the top of the poured HA slurry, lamellar ice crystals with different thickness grew throughout the samples.
Cells were washed in phosphate-buffered saline (PBS), fixed in 4% para-formaldehyde (PFA), permeabilized in 0.01% saponin for 5 min on ice, incubated with different primary antibodies for 1 h, and revealed by appropriate Alexa-Fluor-tagged secondary antibody (Molecular Probes by Life Technologies).
Create your own business plan 1.2 Mission Ice Dreams will produce and sell shave ice with 20 different flavored syrups, soft drinks, and licuados to consumers in El Centro, California.
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