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The presence of genes possibly involved in the transfer of HPI-ICEEh1 were investigated because in previous studies the ICE segment of the HPI-ICE was shown to be necessary for the autonomic integration and circularization of the HPI [7], [10].
Furthermore, the HPI-negative isolates did not contain the ICE segment (or even part of it), supporting the hypothesis that an HPI-ICE is the progenitor of the HPI in Yersinia species [10].
Genes located on the ICE segment of HPI-ICEKp1 that are necessary for excision and conjugation are 99% identical to those from HPI-ICEEc1.
All cluster 3 intB-containing isolates (partly) had an ICE segment, while only a fraction of the cluster 1 intB-containing isolates (partly) harbored an ICE segment, which indicates that the ICE is derived from isolates with the cluster 3 intB gene.
The other segment, which is located after the HPI segment but in front of the putative ICE segment, is similar to part of a large plasmid in K. pneumoniae, and it contains genes responsible for DNA conjugative transfer [7].
BLAST analysis of the ICE segment of HPI-ICE (the variable region of the HPI) of ECOR31 showed that it is most similar to the HPI-ICE of 05-545.
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CEO of Professional Science Editing for Scientists @ prosciediting.com