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New York State Assemblymember Francisco Moya also spoke out against the arrest, calling ICE protocol "unhinged".
The White House did not respond to a request for comment on possible changes to ICE protocol Wednesday afternoon, but that agency did confirm the 2011 policy on avoiding sensitive locations remains active.
The first cycle of chemotherapy (ICE protocol; ifosfamide, carboplatin, etoposide) resulted in a complete remission and the NPM1 mutation was no longer detectable via sequencing.
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Staining was performed in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA) for 30 min on ice using standard protocols.
In comparing the fixed-protocol strategy and the flexible-protocol strategy, both show stabilization of Chao2 and ICE, with the flexible-protocol method indicating lower richness estimates.
The standardized treatment based on the RICE (rest, ice, compression, and elevation) protocol was given to all patients.
The fvERM cells were incubated with antibodies according to the manufacturers' protocol on ice for 30 min then washed again with FACS buffer and fixed in 1% paraformaldehyde (PFA)/phosphate buffered saline (PBS) and analyzed within 1 day.
Joint mobilisation led to increased ankle movement, improved gait patterns, reduced pain and the number of treatment sessions required, and hastened return to activities when compared with the usual rest, ice, compression and elevation protocol [ 35].
Densitometric analysis was performed using imageJ analysis and results were corrected for protein loading by normalization for β-actin expression as described in Singh et al. For cell surface biotinylation, cells were treated as required and incubated for 20 min with 1.5 mg/ml Sulfo-NHS-LC-Biotin (Pierce) in PBS (pH 8.0) on ice and followed the protocol as mentioned before.
It asks us to forget (momentarily) the secular painting that rapidly succeeded it in the public consciousness, Velázquez's Las Meninas, with its dazzling display of tricksy illusionism, bravura brushwork and ice-cool Hapsburg court protocol (and don't dare visit the girl upstairs, the Rokeby Venus).
Following a standard whole cell extraction protocol using ice-cold NP40 buffer, BRCA1 was extensively degraded even in the presence of protease inhibitors (data not shown).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com