The petri dish was placed on ice and irradiated by 1,000 J/m of UV light.
The coverslips were then removed and the slides placed on ice and irradiated (0 5 Gy) as described in section Cell lines and culture.
After RNA-binding, the reaction mixture was placed on ice and irradiated with a UV-lamp (UVP; 800,000 μJ/cm) for 15 min.
Photocrosslinking Proteins (100 μM, if not specifically indicated) containing the UV-inducible crosslinking amino acid p Bpa were incubated in 20 mM NaH2PO4 pH 8.0, 150 mM NaCl on ice and irradiated with UV light (λ = 365 nm) using a BLAK-Ray UVP, Model B 100 AP UV lamp.
Proteins (100 μM, if not specifically indicated) containing the UV-inducible crosslinking amino acid pBpa were incubated in 20 mM NaH2PO4 pH 8.0, 150 mM NaCl on ice and irradiated with UV light (λ = 365 nm) using a BLAK-Ray UVP, Model B 100 AP UV lamp.
An H2O CO2 NH3 = 1 1 1 ice mixture, used as a model mixture for cometary and interstellar ices, was irradiated with ultraviolet (UV /extreme ultraviolet (EUV) photons in the broad 4 20 eV (62 310 nm) energy range at 16 K.
An experiment where a H2O CO2 NH3 = 1 1 1 ice mixture was irradiated using the ultraviolet/extreme ultraviolet (UV/EUV) light provided by a synchrotron beam in the broad 4 20 eV (62 310 nm) range at 16 K is presented here.
Seventy μl of cell suspension (at 100 cells/μl) in a 96-well plate was irradiated on ice in a custom-made ice box described above.
The sample was kept in the dark and incubated on ice for 2 h and then irradiated in an ice bath for 30 min in the photochemical safety cabinet with a mercury arc lamp.
Those agarose plugs were irradiated on ice to prevent DNA repair during irradiation and thereafter directly subjected to cell lysis (0.4 M EDTA, 2% N-lauryl sarcosine, and 1 mg ml−1 proteinase K, all Sigma, Deisenhofen, Germany).
