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The ICE element is necessary for the conjugation and integration of HPI [7].
The EHOS contained a new HPI variant, now termed HPI-ICEEh1, with an ICE element highly similar to the ICE element of HPI-ICEEc1 from E. coli ECOR31 [10] [10].
The EHOS was found to contain a new HPI-ICE variant we termed HPI-ICEEh1, with an ICE element highly similar to the ICE element of HPI-ICEEc1 from E. coli ECOR31 [10].
Altogether, we may conclude that the ICE element is not from a bacterium, but that its nature remains elusive.
This ICE element, found downstream of a glycyl tRNA gene, contains all the necessary genes for mineralization of 3-chlorobenzoate along with an int gene coding for a site-specific integrase and attachment sites (Gaillard et al. 2006).
Also, the presence of SCC mec, an ICE element, a prophage, a transposon, and a plasmid in the MSHR1132 genome demonstrates that this lineage is not completely resistant to genetic invaders.
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Gaps within ICE elements were closed by Sanger sequencing.
Possible integration sites are also located on the ICE elements themselves, and we found several cases where one ICE is integrated into another.
However, in some cases, ICEs have undergone massive expansion, such as the 185 ICE elements in Orientia tsutsugamushi that occupy 35% of the genome [ 87].
Sequences of other ICE elements can be found in GenBank under the strain designations and at the genome positions shown in Table 1.
Unmapped reads were assembled de novo using Velvet [ 39], and ICE elements were identified by BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
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