After three days rats were decapitated in ether anesthesia, brains were quickly dissected over ice to isolate cortex, and visually inspected to verify the injection site.
Mice were decapitated, brains dissected on ice, and frontal cortex and hippocampus were dissected and homogenized in 9 ml of ice-cold buffer (0.32 M sucrose, 4.2 mM HEPES, pH 7.4) with a Teflon-glass homogenizer (Wheaton Science Products, Millville, NJ).
Brains were rapidly removed and lesioned side cortex dissected and frozen on dry ice.
Following anesthesia and perfusion as above, the brain was removed, dissected into hippocampus and cortex on ice and snap-frozen in liquid nitrogen.
Brains were removed and dissected on ice to separate the cortex, hippocampus and the thalamus/hypothalamus before being frozen in liquid nitrogen and stored at −80°C.
For regional protein and mRNA analysis, mice were sacrificed by CO2 asphyxiation, and brains were rapidly removed and both halves dissected on ice into 6 regions (cortex [CTX], subcortex [SUB; includes striatum, thalamus, and hypothalamus], hippocampus [HIPP], midbrain [MID], brainstem [BS], cerebellum [CB]).
In the case of BN-PAGE analysis, brains were quickly dissected on ice and the cerebellum, cortex and hippocampus immediately frozen on dry ice and stored at −80°C.
Accumulation of large ice crystals in the cortex may arise partly from cortical tissues having very large vacuoles even in December.
It may also help retain the icicles by promoting recrystallization in the cortex as ice management to avoid freeze desiccation of the tissues, which is only hypothetical at the moment.
We then extracted brains and dissected them on ice into hypothalamus (HYP), prefrontal cortex (PFC), hippocampus (HIPP), and adjacent parietal cortex (PCX).
